EFFECT OF LIPOPOLYSACCHARIDE AND DEXAMETHASONE ON CULTURAL PROPERTIES OF MULTIPOTENT STROMAL CELLS FROM BONE MARROW, THYMUS, AND ADIPOSE TISSUE OF MICE

Nikolskiy I.S.¹, Nikolska V.V. ¹, Semenova Ya.-M.O. ¹, Taranukha L.I.¹, Podpryatov S.S.²

• ¹State Institution «National Scientific Center ‘M.D. Strazhesko Institute of Cardiology, Clinical and Regenerative Medicine’ of the National Academy of Medical Sciences of Ukraine», Kyiv
• ²Kyiv City Clinical Hospital No. 1, Kyiv

Summary. The objective: to investigate the cultural, clonogenic, and secretory properties of multipotent stromal cells (MSCs) derived from bone marrow, thymus, and adipose tissue of mice, and to assess their response to lipopolysaccharide (LPS) and dexamethasone (DM). Materials and methods. MSCs were isolated from femoral bone marrow, thymus, and subcutaneous adipose tissue of adult non-inbred mice using flushing, explant, and enzymatic dissociation methods, respectively. Proliferative activity was evaluated by determining population doubling time (PDT), and clonogenic efficiency was assessed by the number of colony-forming unit-fibroblasts (CFU-F). The ability of MSCs for contact interaction was determined by fibroblast-lymphocyte rosette (FLR) formation, and cytokine secretion (IL-6, IL-10) was measured in culture supernatants. Cells were incubated with LPS (0.1 μg/mL), DM (0.1 μg/mL), or their combination. Statistical analysis was performed using the nonparametric Mann–Whitney test. Results. LPS activated proliferation and clonogenic activity of MSCs, enhanced FLR formation, and stimulated IL-6 secretion, while dexamethasone exerted mainly inhibitory effects on proliferation and reduced IL-6 production, maintaining IL-10 levels. The highest clonogenic potential, proliferative rate, and cytokine activity were observed in thymic and adipose-derived MSCs. Conclusions. The results demonstrate tissue-specific differences in the biological properties of MSCs from different sources and confirm the multidirectional influence of LPS and dexamethasone on their functional activity. The revealed regularities may be useful for developing cell-based therapeutic products aimed at controlling systemic inflammation and promoting regenerative processes.